Activity of Adenylate Cyclase in Embryonic Cell

A total of 10 normal cases for each pregnancy month, from the fifth month, were randomly selected, in Mosul Albatool hospital. Also 3 cases of abnormal embryos were chosen for each month, from the seventh month of pregnancy. Amniotic fluids was collected, by trans-vaginal method amniocentesis. The embryonic cells were separated and extracted; the resulted pellets were used for adenylate cyclase determination. While cyclic AMP measured in both cell filtrate and the amniotic fluids. The result yield that cyclic AMP decreases, from 50 g /l. to 17 g/l. in amniotic fluid, and from 32g/l. to 16 g/l. in embryonic cell through the last three month of pregnancy. Also the results showed an increase in cyclic AMP, in abnormal pregnancy comparing with the normal cases while a decreases through pregnancy advancing in the last three month in both amniotic fluid and embryonic cell from 62g/l to 59g/l and from 44g/l to 42g/l respectively. While the activity of adenylate cyclase decrease in relative to the cyclic AMP level, yielding 32.234 mol/min/mg enzyme to 11,231mol/min/mg enzyme, in normal embryonic cell and from 22.245 mol/min/mg to 21.231 mol/min/mg enzyme in abnormal embryonic cell. While protein did not change significantly in embryonic cell in both normal and abnormal cases.